A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT₃ Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

Table 6

Effects of HPLC column, flow rate, pH value of mobile phase, and column temperature upon RCF of the five 5-HT₃ receptor antagonists.

Compound	RCF	RSD (%)
		Instrument	Column	Flow rate	Temperature	pH value
Azasetron	0.340	0.9	2.8	1.6	0.3	2.1
Granisetron	0.374	1.2	3.0	1.0	0.6	1.7
Tropisetron	0.572	0.8	2.3	1.5	1.2	2.2
Ondansetron	1.000	0	0	0	0	0
Ramosetron	1.247	1.5	4.2	1.3	0.8	2.5