Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Vardenafil and Its Application of Bioequivalence

Figure 3

Chromatogram for (a) blank plasma with no interfering peaks at the retention time of the analytes were observed; (b) blank plasma spiked with vardenafil at LLOQ 0.5 ng/mL and IS at 40 ng/mL; (c) blank plasma spiked with vardenafil at ULOQ 60 ng/mL and IS at 40 ng/mL; and (d) plasma sample in rabbit 1.5 h after drug administration with a concentration of 44.95 ng/mL.

(a)

(b)

(c)

(d)