Determination of Dabigatran Concentration in Human Plasma and Breast Milk

Table 1

Gradient programme on the Dionex Ultimate HPLC system using an Ace 5 C8-300 column (250 mm × 1 mm).

Minutes	Flow (μL/min)	% B	% C
0.0	50	4	0
1.5	50	4	0
2.5	50	15	0
10.0	50	40	0
12.5	50	65	0
14.0	50	95	0
14.1	50	95	0
14.2	50	4	0
15.0	50	4	96
15.8	50	4	96
17.0	50	4	0
25.0	50	4	0