International Journal of Analytical Chemistry

Research Article

Rapid Identification and Systematic Mechanism of Flavonoids from Potentilla freyniana Bornm. Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Network Pharmacology

Table 1

The retention time and mass spectrometric data of flavone in Potentilla freyniana Bornm.


Peak	t_R	Theoretical mass, m/z	Experimental mass, m/z	Error (ppm)	Formula	MS/MS fragment	Identification

1	3.59	465.1038	465.1039	0.21	C₂₁H₂₂O₁₂	MS²[465]: 285.0406(100), 125.0233(72), 275.0563(45), 177.0185(29), 303.0512(20), 151.0032(17)	Taxifolin-glucoside
2	3.72	465.1038	465.1033	−1.08	C₂₁H₂₂O₁₂	MS²[465]: 285.0405(100), 125.0233(38), 273.0043(28), 177.0185(19), 303.0511(15)	Taxifolin-glucoside
3	3.88	465.1038	465.1042	0.86	C₂₁H₂₂O₁₂	MS²[465]: 285.0405(100), 125.0233(36), 177.0184(18), 275.0566(12), 303.0512(10)	Taxifolin-glucoside
4	4.13	463.0882	463.0881	−0.22	C₂₁H₂₀O₁₂	MS²[463]: 287.0562(100), 259.0612(78), 125.0232(32)	Eriodictyol-glucuronide
5	4.19	449.1089	449.1093	0.89	C₂₁H₂₂O₁₁	MS²[449]: 259.0611(100), 287.0561(33), 178.9979(15), 125.0231(13)	Eriodictyol-glucoside
6	4.41	593.1512	593.1535	3.88	C₂₇H₃₀O₁₅	MS²[593]: 353.0667(100), 383.0774(54), 473.1092(35), 125.0234(25), 413.0874(6)	Vicenin II
7	4.56	449.1089	449.1092	0.67	C₂₁H₂₂O₁₁	MS²[449]: 259.0612(100), 269.0455(76), 287.0563(44), 125.0233(38), 178.9977(22)	Eriodictyol-glucoside
8	5.61	465.1038	465.1042	0.86	C₂₁H₂₂O₁₂	MS²[465]: 125.0233(100), 285.0405(62), 259.0610(48), 275.0566(32), 303.0512(18)	Taxifolin-glucoside
9	5.75	479.0831	479.0834	0.63	C₂₁H₂₀O₁₃	MS²[479]: 285.0405(100), 125.0233(41), 303.0512(18), 177.0186(17), 169.0133(15), 259.0613(14)	Taxifolin-glucuronide
10	5.88	449.1089	449.1092	0.67	C₂₁H₂₂O₁₁	MS²[449]: 269.0456(100), 151.0026(69), 178.9979(39), 125.0231(13), 259.0612(12), 287.0562(10)	Eriodictyol-glucoside
11	6.12	433.1140	433.1142	0.46	C₂₁H₂₂O₁₀	MS²[433]: 271.0614(100), 151.0027(73). 119.0491(18), 125.0229(8)	Naringenin-glucoside
12	6.22	625.1408	625.1408	0.00	C₂₇H₃₀O₁₇	MS²[625]: 287.0558(100), 113.0231(40), 151.0030(38)	Eriodictyol-glucoside-glucuronide
13	6.57	303.0510	303.0507	−0.99	C₁₅H₁₂O₇	MS²[303]: 125.0234(100), 285.0407(38)	Taxifolin
14	6.59	433.1140	433.1141	0.23	C₂₁H₂₂O₁₀	MS²[433]: 271.0613(100), 151.0027(72), 119.0491(16)	Naringenin-glucoside
15	6.85	463.0882	463.0886	0.86	C₂₁H₂₀O₁₂	MS2[463]: 300.0272(100), 301.0350(70)	Hyperoside
16	6.90	449.1089	449.1094	1.11	C₂₁H₂₂O₁₁	MS²[449]:151.0028(100), 287.0562(73), 135.0442(32)	Eriodictyol-glucoside
17	7.18	463.0882	463.0886	0.86	C₂₁H₂₀O₁₂	MS2[463]: 300.0279(100), 301.0358(50), 151.0025(8), 178.9976(7)	Isoquercitrin
18	7.75	597.1825	597.1832	1.17	C₂₇H₃₄O₁₅	MS²[597]: 357.0983(100), 387.1087(85), 315.0882(22), 417.1165(21), 358.1008(15)	Phloretin-C- diglucoside
19	7.99	449.1089	449.1085	−0.89	C₂₁H₂₂O₁₁	MS²[449]: 167.0340(69), 137.0233(50), 123.0441(12)	Phloretin-glucuronide
20	8.15	431.0984	431.0992	1.86	C₂₁H₂₀O₁₀	MS²[431]: 269.0445(100)	Baicalein-glucoside
21	9.60	431.0984	431.0988	0.93	C₂₁H₂₀O₁₀	MS²[431]: 269.0443(100)	Baicalein-glucoside
22	9.61	449.1089	449.1086	−0.67	C₂₁H₂₂O₁₁	MS²[449]: 151.0026(100), 287.0563(65), 135.0442(22)	Eriodictyol-glucoside
23	9.68	565.1563	565.1568	0.88	C₂₆H₃₀O₁₄	MS²[565]: 271.0614(100), 151.0026(48)	Naringenin-pentoside-glucoside
24	9.82	433.1140	433.1142	0.46	C₂₁H₂₂O₁₀	MS²[433]: 271.0615(100), 151.0028(41), 119.0491(14)	Naringenin-glucoside
25	10.38	431.0984	431.0992	1.86	C₂₁H₂₀O₁₀	MS²[431]: 268.0369(100), 269.0444(47)	Apigenin-7-glucoside
26	11.18	463.0882	463.0886	0.86	C₂₁H₂₀O₁₂	MS²[463]: 151.0027(100), 113.0231(66), 287.0562(52), 161.0234(40), 337.0569(39), 135.0439(23)	Eriodictyol-glucuronide
27	12.16	435.1297	435.1300	0.69	C₂₁H₂₄O₁₀	MS²[435]: 273.0769(100), 167.0340(69), 125.00232(9), 179.0341(6)	Phlorizin
28	12.36	477.0674	477.0678	0.84	C₂₁H₁₈O₁₃	MS²[477]: 301.0353(100), 151.0029(11), 178.9983(9)	Quercetin-glucuronide
29	12.51	431.0984	431.0986	0.46	C₂₁H₂₀O₁₀	MS²[431]: 268.0371(100), 269.0441(18), 239.0337(9)	Apigenin-4′-glucoside
30	12.62	477.0674	477.0678	0.84	C₂₁H₁₈O₁₃	MS²[477]: 301.0354(100), 178.9983(12), 151.0029(8)	Quercetin-glucuronide
31	13.00	433.1140	433.1144	0.92	C₂₁H₂₂O₁₀	MS²[433]: 271.0612(100), 151.0027(46)	Naringenin-glucoside
32	13.39	567.1719	567.1730	1.94	C₂₆H₃₂O₁₄	MS²[567]: 273.0771(100), 167.0340(42), 125.0020(22)	Phloretin-pentoside-glucoside
33	13.45	287.0561	287.0564	1.05	C₁₅H₁₂O₆	MS2[287]: 151.0027(100), 135.0441(69), 107.0125(10)	Eriodictyol
34	13.57	449.1089	449.1085	−0.89	C₂₁H₂₂O₁₁	MS²[449]: 137.0233(100), 167.0340(89), 123.0440(8)	Phloretin-glucuronide
35	13.67	435.1297	435.1303	1.38	C₂₁H₂₄O₁₀	MS²[435]: 273.0767(100), 167.0339(52)	Trilobatin
36	13.86	301.0354	301.0356	0.66	C₁₅H₁₀O₇	MS²[301]: 151.0027(100), 178.9978(66), 121.0284(21), 107.0125(8)	Quercetin
37	13.95	285.0405	285.0407	0.70	C₁₅H₁₀O₆	MS²[285]: 285.0404(100), 151.0033(12), 133.0290(11), 175.0395(8)	Luteolin
38	14.79	271.0611	271.0614	1.11	C₁₅H₁₂O₅	MS²[271]: 151.0027(100), 119.0499(36), 177.0184(14), 93.0333(14), 107.0126(9)	Naringenin
39	14.95	269.0455	269.0454	−0.37	C₁₅H₁₀O₅	MS²[269]: 269.0455(100), 117.0344(8), 149.0239(7), 151.0035(7)	Apigenin
40	14.99	273.0768	273.0771	1.10	C₁₅H₁₄O₅	MS²[273]: 167.0341(100), 123.0438(18), 119.0491(12),125.0231(8)	Phloretin
41	15.07	285.0405	285.0405	0.00	C₁₅H₁₀O₆	MS²[285]: 285.0403(100), 151.0030(5)	Kaempferol
42	15.49	269.0455	269.0453	−0.74	C₁₅H₁₀O₅	MS²[269]: 269.0453(100), 241.0504(9), 251.0348(8), 223.0399(7)	Baicalein
43	16.71	283.0612	283.0613	0.35	C₁₆H₁₂O₅	MS²[283]: 268.0376(100), 163.0035(7)	Wogonin

Identified by comparing with reference standards.