Simultaneous Quantification of Aspirin, Its Metabolite Salicylic Acid, and Salvianolic Acid B in Human Plasma Using UPLC-MS/MS

Table 3

Extraction recovery and matrix effects of Sal B, ASA, SA, and IS (n = 6).

Compound	Nominal conc. (ng/mL)	Extraction recovery (mean ± SD%)	ME (mean ± SD%)	IS-normalized ME (mean ± SD%)	RSD% (IS-normalized ME)
Sal B	15	83.3 ± 4.3	101 ± 4	92.1 ± 4.3	3.95
	3000	85.3 ± 4.3	103 ± 4	94.0 ± 2.8
	4500	87.2 ± 4.9	99.7 ± 4.0	91.3 ± 3.9
ASA	15	97.9 ± 5.2	102 ± 3	93.4 ± 3.0	3.75
	3000	96.3 ± 2.6	97.3 ± 3.2	93.3 ± 3.1
	4500	93.2 ± 1.8	101 ± 2	92.3 ± 1.7
SA	15	93.1 ± 4.3	106 ± 8	97.1 ± 7.0	4.55
	3000	94.6 ± 1.3	96.7 ± 2.6	95.0 ± 2.5
	4500	95.2 ± 2.1	96.7 ± 2.1	95.0 ± 2.6
IS	20	107 ± 6	109 ± 3	—

Extraction recovery = (mean peak area of analyte in the plasma matrix)/(mean peak area of analyte in the extracted plasma matrix). ME = (mean peak area of analyte in the extracted plasma matrix)/(mean peak area of analyte in the dilution solution). IS-normalized ME = ((mean peak area of analyte in the extracted plasma matrix)/(mean peak area of analyte in the dilution solution))/((mean peak area of IS in the extracted plasma matrix)/(mean peak area of IS in the dilution solution)). RSD% = (standard deviation/mean) × 100%.