International Journal of Analytical Chemistry / 2021 / Article / Tab 2 / Review Article
Point-of-Care Diagnoses and Assays Based on Lateral Flow Test Table 2 LFT signal recording by an analytical instrument.
Type of assay Type of instrumentation Physical principle of the instrumental assay Reference LFT for p24 protein from human immunodeficiency virus, gold nanoparticles conjugated with a monoclonal antibody against p24 were used Laser alighting specific spots, IR camera visually recording temperature Thermal contrast reading [41 ] Detection of 16S rRNA gene from Escherichia coli by hybridization and use of biotinylated probe and peroxidase conjugated with streptavidin, peroxidase was responsible for the chemiluminescent reaction Digital camera with CMOS chip Digital camera recorded chemiluminescence provided by peroxidase [76 ] Detection of fumonisin, labeling of antibodies by peroxidase allows performing chemiluminescent chemical reaction that is instrumentally measured Digital camera with CCD chip Digital camera recorded chemiluminescence provided by peroxidase [77 ] LFT immunoassay of progesterone based on labeling by carbon colloid Digital scanner Strips were scanned and digital data analyzed [78 ] The LFT assay comprised of the use of streptavidin-biotinylated DNA probes modified with gold nanoparticles Smartphone camera Detected spots were photographed by a smartphone camera, and coloration was measured [79 ] LFT where coloration was initiated Prussian blue nanoparticles as artificial nanozymes Smartphone camera Detected spots were photographed by a smartphone camera, and coloration was measured [80 ]