Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Table 1

Molecular binding ratio of ZEN-BSA prepared via the four methods.

Synthesis methods	Initial molar ratio of ZEN to BSA	Molecular binding ratio of ZEN-BSA	Usage percentage of ZEN^∗ (%)
OAE	50 ∶ 1	17.2 ∶ 1	34.4
CMA	50 ∶ 1	14.6 ∶ 1	29.2
FA	50 ∶ 1	9.7 ∶ 1	19.4
BDE	50 ∶ 1	8.3 ∶ 1	16.6

^∗Since the molecular weight of BSA (66446) was much larger than that of ZEN (318.36), the utilization rate of BSA was set to 100% when calculating the utilization rate of BSA and ZEN.