Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Table 3

The cross-reaction of ZEN pAb with ZEN and its analogs.

Compound	ZEN pAb (OAE)		ZEN pAb (CMA)		ZEN pAb (FA)		ZEN pAb (BDE)
	IC50 (μg/L)^a,b	CR (%)^a	IC50 (μg/L)^a,b	CR (%)^a	IC50 (μg/L)^a,b	CR (%)^a	IC50 (μg/L)^a,b	CR (%)^a
ZEN	11.67	100	16.29	100	20.92	100	24.36	100
α-ZAL	31.95	36.53	69.17	23.55	2682.05	0.78	1441.42	1.69
β-ZAL	68.73	16.98	133.74	12.18	2582.72	0.81	2511.34	0.97
α-ZOL	18.14	64.33	31.41	51.86	3670.18	0.57	990.24	2.46
β-ZOL	57.89	20.16	87.49	18.62	4358.33	0.48	1331.15	1.83
ZON	109.48	10.66	168.98	9.64	2273.91	0.92	66.61	36.57

^aAll of the data were calculated from triplicate assays. ^bThe compound standard solution was prepared in 70% methanol-PBS (7 : 3, v/v).