Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Table 4

Comparison of IC50 values and CR values of ZEN pAbs by the OAE method and FA method in this study and other previously reported ZEN pAbs and ZEN mAbs.

References	ZEN antibody	Coupling method	Immunoassay format	IC50 of ZEN (μg/L)	CR (%)^a
					α-ZAL	β-ZAL	α-ZOL	β-ZOL	ZON
This study (2021)	ZEN pAb	OAE	icELISA^b	11.67	36.53	16.98	64.33	20.16	10.66
Dong et al. [49]	ZEN mAb 6C2	OAE	icELISA	114.0	99.2	55.5	89.5	39.3	—
Zhang et al. [23]	ZEN mAb 3D4	OAE	FPIA^c	0.041	107.9	57.2	103.5	73.9	66
Liu et al. [50]	ZEN mAb 7B2	OAE	BA-ELISA^d	0.18	46.7	39.2	60.5	24.7	59.5
Cha et al. [27]	ZEN mAb kk-ZEN	OAE	icELISA	131.3	108.1	119.3	114.1	130.3	—
			dcELISA^e	150.6	123.8	99.3	149.5	124.8	—
Thongrussamee et al. [51]	ZEN mAb 2C5	OAE	dcELISA	1.79	121.5	65.3	21.5	18.9	—
This study (2021)	ZEN pAb	FA	icELISA	20.92	0.78	0.81	0.57	0.48	0.92
Burkin et al. [52]	ZEN pAb	FA	icELISA	31.7	0.12	—	0.15	0.02	—
Gao et al. [29]	ZEN pAb	FA	icELISA	233.35	2.25	5.65	3.14	1.96	6.79
	ZEN mAb^#	FA	icELISA	55.72	0.63	0.92	0.65	0.94	1.48

^aAll of the data were calculated using the CR of ZEN as 100%. ^bicELISA: indirect competitive enzyme-linked immunosorbent assay; ^cFPIA: fluorescence polarization immunoassay; ^dBA-ELISA: biotin-streptavidin amplified enzyme-linked immunosorbent assay; ^edcELISA: direct competitive enzyme-linked immunosorbent assay; —: no data. ^#No name.