HPLC-MS/MS-Mediated Analysis of the Pharmacokinetics, Bioavailability, and Tissue Distribution of Schisandrol B in Rats

Table 2

Precision and accuracy of the approach to Schisandrol B measurement in biological samples (n = 6).

Samples	Spiked conc (ng/mL)	Intra-day (n = 6)			Inter-day (n = 6 × 3)
		Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (RE, %)	Measured conc. (ng/mL)	Precision (RSD, %)	Accuracy (RE, %)
Plasma	5	5.46 ± 0.26	4.73	9.22	5.45 ± 0.27	4.99	8.99
	15	13.60 ± 1.40	10.32	−9.31	13.68 ± 1.43	10.46	−8.78
	150	145.30 ± 13.05	8.98	−3.14	143.58 ± 14.11	9.82	−4.28
	1500	1436.42 ± 93.55	6.51	−1.08	1410.53 ± 112.67	7.99	−5.96
Heart	1	0.99 ± 0.08	7.91	−1.27	0.97 ± 0.03	3.08	−3.41
	2	2.00 ± 0.22	10.82	0.17	2.01 ± 0.02	0.96	0.50
	40	36.90 ± 0.39	1.06	−7.74	37.11 ± 0.22	0.59	−7.22
	800	789.33 ± 48.32	6.12	−1.33	753.10 ± 32.12	4.27	−5.86
Liver	1	0.98 ± 0.02	2.47	−2.16	0.96 ± 0.06	6.50	−4.06
	2	1.83 ± 0.06	3.11	−8.49	1.85 ± 0.02	0.90	−7.53
	40	38.32 ± 0.96	2.51	−4.20	38.09 ± 0.21	0.55	−4.78
	800	713.49 ± 7.44	1.04	−10.81	735.12 ± 18.94	2.58	−8.11
Spleen	1	0.96 ± 0.03	3.24	−4.17	0.95 ± 0.03	2.66	−4.52
	2	1.90 ± 0.10	5.02	−5.17	1.85 ± 0.05	2.80	−7.31
	40	40.18 ± 1.77	4.42	0.45	39.38 ± 0.78	1.97	−1.54
	800	761.79 ± 53.22	6.99	−4.48	762.48 ± 9.57	1.26	−4.69
Lung	1	1.00 ± 0.10	10.02	0.10	1.01 ± 0.02	1.52	0.72
	2	2.06 ± 0.10	4.82	2.82	2.03 ± 0.06	3.12	1.41
	40	36.27 ± 2.27	6.26	−9.32	37.01 ± 0.88	2.37	−7.47
	800	722.25 ± 43.09	5.97	9.72	723.66 ± 4.49	0.62	−9.54
Kidney	1	0.90 ± 0.05	6.08	−10.33	0.97 ± 0.07	6.96	−2.93
	2	1.85 ± 0.11	6.06	−7.70	1.88 ± 0.04	2.08	−5.86
	40	41.92 ± 3.88	9.26	4.81	40.94 ± 0.88	2.15	2.36
	800	721.96 ± 30.57	4.23	9.76	748.65 ± 25.22	3.37	−6.42
Brain	1	1.03 ± 0.06	6.25	2.73	0.98 ± 0.05	5.10	−2.02
	2	2.04 ± 0.06	3.07	1.95	1.89 ± 0.14	7.31	−5.47
	40	43.12 ± 2.37	5.51	7.81	39.06 ± 3.55	9.09	−3.35
	800	749.13 ± 31.01	4.14	−6.36	736.89 ± 13.01	1.77	−7.89