HPLC-MS/MS-Mediated Analysis of the Pharmacokinetics, Bioavailability, and Tissue Distribution of Schisandrol B in Rats

Table 4

Stability of Schisandrol B in rat plasma and tissues homogenate samples under various conditions (n = 6).

Samples	Spiked conc. (ng/mL)	Measured conc.
		Bench-top (for 4 h)	Autosampler (at room temperature for 24 h)	Three freeze-thaw cycles (at −80°C)	Long term (at −80°C for 20 days)
Plasma	15	13.95 ± 1.21	14.04 ± 1.21	13.70 ± 0.98	13.92 ± 0.96
	1500	1345.92 ± 97.42	1433.13 ± 111.16	1333.92 ± 58.09	1387.98 ± 72.00
Heart	2	2.00 ± 0.25	2.03 ± 0.13	1.80 ± 0.09	1.91 ± 0.16
	800	728.09 ± 12.10	741.90 ± 66.63	743.67 ± 44.47	732.25 ± 32.14
Liver	2	1.86 ± 0.05	1.86 ± 0.04	1.81 ± 0.06	1.82 ± 0.21
	800	748.70 ± 10.92	743.18 ± 18.64	761.94 ± 36.57	756.91 ± 42.36
Spleen	2	1.80 ± 0.01	1.87 ± 0.07	1.85 ± 0.11	1.84 ± 0.09
	800	753.27 ± 8.88	772.37 ± 10.61	781.57 ± 22.05	764.19 ± 25.38
Lung	2	1.96 ± 0.24	2.07 ± 0.16	1.89 ± 0.07	1.93 ± 1.98
	800	728.69 ± 21.07	720.05 ± 15.93	777.29 ± 42.83	748.07 ± 38.92
Kidney	2	1.92 ± 0.25	1.88 ± 0.11	1.88 ± 0.04	1.86 ± 0.17
	800	772.08 ± 88.79	751.92 ± 50.02	773.92 ± 47.36	766.91 ± 50.09
Brain	2	1.77 ± 0.19	1.87 ± 0.12	1.98 ± 0.04	1.80 ± 0.11
	800	723.23 ± 57.23	738.33 ± 8.57	732.87 ± 49.33	729.24 ± 37.43