A Rapid Screening Method for Sibutramine Hydrochloride in Natural Herbal Medicines and Dietary Supplements
Table 2
The analysis results of the orthogonal experiment.
No.
V1 (ml)
C1 (μg/ml)
Extraction rate (%)
V2 (ml)
V2 : V3
V3 (ml)
C2 (μg/ml)
LLE rate (%)
Transfer efficiency (%)
1
7
16.59
87.74
4.0
1 : 3
12.0
5.36
99.03
86.89
2
2 : 3
6.0
10.63
94.94
83.30
3
4 : 3
3.0
18.88
91.27
80.08
4
3.5
30.91
81.67
2.0
1 : 3
6.0
9.97
95.80
78.24
5
2 : 3
3.0
20.23
104.64
85.46
6
4 : 3
1.5
41.00
103.17
84.26
7
2.0
29.84
43.30
1.0
1 : 3
3.0
17.84
179.33
77.65
8
2 : 3
1.5
23.85
119.90
51.92
9
4 : 3
0.75
48.85
122.78
53.16
C: the concentration of sibutramine hydrochloride in the detection solution. LLE rate: the extraction rate of liquid-liquid extraction step. Transfer efficiency: the efficiency of the sibutramine transfer from the sample to the test liquid.
