Comparison of the Saponins in Three Processed American Ginseng Products by Ultra-High Performance Liquid Chromatography-Quadrupole Orbitrap Tandem Mass Spectrometry and Multivariate Statistical Analysis

Table 1

Compounds identified from American ginseng by natural drying, steam drying, and vacuum freeze-drying.

No.	Rt (min)	Identification	Formula	Detected mass (Da)	Mass error (ppm)
1	5.67	Vina-ginsenoside R4	C48H82O19	1007.5457^a	2.5
2	9.04	Notoginsenoside R1	C47H80O18	977.5346^a	1.9
3	10.03	Ginsenoside Rg1	C42H72O14	845.4914^a	1.2
4	10.41	Ginsenoside Re	C48H82O18	991.5504^a	2.1
5	10.73	24 (S)-pseudo-ginsenoside F11	C42H72O14	845.4922^a	2.1
6	11.45	Malonyl-ginsenoside Rg1	C45H74O17	885.4867^b	1.6
7	11.51	Malonyl-ginsenoside Re	C51H84O21	1031.5457^b	2.4
8	12.69	20 (S)-notoginsenoside R2	C41H70O13	815.4819^a	2.6
9	13.47	Ginsenoside F5	C41H70O13	815.4814^a	2.0
10	13.85	Acetyl-Rg1	C44H74O15	887.5024^a	1.6
11	14.80	Pseudo-RT2	C41H70O14	785.4661^b	4.1
12	15.26	24 (R)-pseudo-ginsenoside F11	C42H72O14	845.4918^a	1.6
13	15.72	Notoginsenoside R4/Ginsenoside Ra3	C59H100O27	1285.6464^a	1.6
14	16.46	Ginsenoside Rh1	C36H62O9	683.4391^a	2.2
15	16.61	20 (S)-ginsenoside Rg2	C42H72O13	829.4971^a	1.9
16	16.89	20 (R)-ginsenoside Rg2	C42H72O13	829.4974^a	2.3
17	17.01	Ginsenoside Rb1	C54H92O23	1153.603^a	1.6
18	17.06	Malonyl-ginsenoside Rb1/Isomer	C57H94O26	1193.5983^b	1.8
19	17.13	Ginsenoside Rc	C53H90O22	1123.5920^a	1.2
20	17.20	Ginsenoside Ro	C48H76O19	955.4933^b	2.6
21	17.27	Malonyl-ginsenoside Rc	C56H92O25	1163.5883^b	2.4
22	17.29	Malonyl-ginsenoside Rb2	C56H92O25	1163.5892^b	3.2
23	17.36	Ginsenoside Rb2	C53H90O22	1123.5923^b	1.5
24	17.59	Ginsenoside Rb3	C53H90O22	1123.5924^a	1.6
25	17.66	Malonyl-ginsenoside Rb3	C56H92O25	1163.5878^b	2.0
26	17.73	Pseudo-RT1	C47H74O18	971.4800^a	5.9
27	17.84	Ginsenoside Rd	C48H82O18	991.5506^a	2.3
28	18.07	Chikusetsusaponin IVa	C42H66O14	793.4396^b	2.0
29	18.10	Malonyl-ginsenoside Rd	C51H84O21	1031.5458^b	2.5
30	18.14	Gypenoside XVII	C48H82O18	991.5502^a	1.9
31	18.40	Pseudo-RC1	C50H48O19	1033.5521^a	6.6
32	18.63	Quinquefolium III	C50H48O19	1033.5529^a	5.8
33	18.80	Ginsenoside Rg6	C42H70O12	811.4810^a	4.8
34	18.90	Ginsenoside Rg4	C42H70O12	811.4807^a	5.2
35	19.24	Ginsenoside F2	C42H72O13	829.4971^a	1.9
36	19.58	20 (S)-ginsenoside Rg3	C42H72O13	829.4976^a	2.5
37	19.63	Ginsenoside Rk3	C36H60O8	665.4231^a	5.9
38	19.79	Ginsenoside Rh4	C36H60O8	665.4233^a	5.6
39	20.01	Zingibroside R1	C42H66O14	839.4382^a	5.1
40	20.27	20 (R)-ginsenoside Rg3	C42H72O13	829.4982^a	3.3
41	20.34	20 (S)-ginsenoside Rs3	C44H74O14	871.5015^a	5.3
42	20.60	Calenduloside E	C36H56O9	677.3869^a	5.5
43	20.68	20 (R)-ginsenoside Rs3	C44H74O14	871.5014^a	5.3
44	20.88	Ginsenoside Rk1	C42H70O12	811.4859^a	1.2
45	20.92	Ginsenoside Rg5	C42H70O12	811.4868^a	2.3
46	21.19	20 (S)-ginsenoside Rh2	C36H62O8	667.4391^a	5.4
47	21.42	20 (R)-ginsenoside Rh2	C36H62O8	667.4393^a	5.1
48	21.62	Ginsenoside Rs5	C44H72O13	853.4913^a	4.7
49	21.85	Ginsenoside Rs4	C44H72O13	853.4913^a	4.7
50	23.18	Ginsenoside Rk2	C36H60O7	649.4276^a	6.9
51	23.33	Ginsenoside Rh3	C36H60O7	649.4294^a	4.2