An Integrated Strategy of Chemical Fingerprint and Network Pharmacology for the Discovery of Efficacy-Related Q-Markers of Pheretima

Table 1

Optimization of the results of the orthogonal experimental design.

No.	Ratio of sample to liquid (A, mg/mL)	Extraction time (B, min)	Extraction temperature (C, °C)	Extraction power (D, W)	Total content (mg/g)
1	10	40	40	220	3.6466
2	10	50	50	290	3.6795
3	10	60	60	360	3.7052
4	20	40	50	360	3.1518
5	20	50	60	220	3.1720
6	20	60	40	290	3.1203
7	30	40	60	290	3.0463
8	30	50	40	360	2.8356
9	30	60	50	220	3.0658
K1	3.677	3.282	3.201	3.295
K2	3.148	2.229	3.299	3.282
K3	2.983	3.297	3.308	3.231
R	0.694	0.068	0.107	0.064
Order	A > C > B > D
Optimal level	10	60	60	220
Optimal combination	10 mg/mL, 60 min, 60°C, 220 W

Note: Ki: the average of each level in each factor; R: the difference between the maximum and minimum levels of each factor.