A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification

Figure 1

Comparison of Linker A/B (MERS 1a and MERS 1b). Experimental conditions: Linker A/B 10 pM, 0.8 mM dNTP, 4 U SplintR Ligase, 46 nM FIP and BIP primers, and 8 U Bst 2.0 DNA polymerase. Detection was performed as described in material and methods.