A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification

Figure 2

Comparison of amplification reaction steps for miRNA-LAMP assay. (a and b) Real-time fluorescence intensity, (c) Ct value for one or two amplification reaction steps methods. Experimental conditions: Linker A/B 10 pM, 0.8 mM dNTP, 4 U SplintR Ligase, 46 nM FIP and BIP primers, and 8 U Bst 2.0 DNA polymerase. Detection was performed as described in material and methods.

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