A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification

Figure 3

Comparison of different ligase enzymes. (a) SplintR Ligase, (b) T4 DNA Ligase, and (c) Taq DNA ligase were analyzed for the real-time fluorescence intensity, (d) Ct value for three enzymes. Experimental conditions: Linker A/B 10 pM, 0.8 mM dNTP, 4 U SplintR Ligase, 46 nM FIP and BIP primers, and 8 U Bst 2.0 DNA polymerase. Detection was performed as described in material and methods.

(a)

(b)

(c)

(d)