A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification

Figure 4

The selectivity of the analysis of miR-200a/mismatch miRNA. Experimental conditions: Linker A/B 10 pM, 0.8 mM dNTP, 4 U SplintR Ligase, 46 nM FIP and BIP primers, 8 U Bst 2.0 DNA polymerase. Detection was performed as described in material and methods.