Rapid Separation of Asiatic Acid, Quercetin, and Kaempferol from Traditional Chinese Medicine Centella asiatica (L.) Urban Using HSCCC-Semi-Prep-HPLC and the Assessment of Their Potential as Fatty Acid Synthase Inhibitors

Table 2

Screening of the solvent system for AA.

Serial number	Solvent system	Component ratio (V : V : V : V)
1	A-1	1 : 1 : 1 : 1
2	A-2	1 : 1.1 : 1 : 1
3	A-3	1 : 1.1 : 0.9 : 1
4	A-4	1.5 : 1 : 0.9 : 1
5	A-5	1.5 : 1 : 0.8 : 1
6	B-1	1.1 : 1 : 1.1 : 1
7	B-2	1.5 : 1 : 0.8 : 1
8	C-1	1 : 1 : 1 : 1
9	C-2	1.5 : 1 : 0.9 : 1
10	C-3	1.8 : 1 : 0.8 : 1

Solvent system A: n-hexane-n-butanol-methanol-water. Solvent system B: n-hexane-n-butanol-ethanol-water. Solvent system C: n-hexane-n-butanol-acetonitrile-water.