Research Article

Evaluation of BACE1 Silencing in Cellular Models

Table 2

List of genes selected for expression level evaluation in HCN A94 cells transfected with BACE1-specific si-5b and si-6, coded in lentivirus vector.

NoProtein Name/Function% of silencing with Lv-si-8% of silencing with Lv-si-9

1BACE1Beta-site APP cleaving enzyme 1 70 75
2BACE2Beta-site APP cleaving enzyme 2
3SCG10Superior cervical ganglion-107070
4NeuroD1Neurogenic differentiation 1
5GluR2Glutamate receptor 2
6CREBcAMP responsive element binding protein 1
7MeCP2Methyl CpG binding protein 2
8Synapsin 1Protein involved in the regulation of neurotransmitter release at synapses
9PKRDouble stranded RNA-dependent protein kinase
10GAPDHGlyceraldehyde-3-phosphate dehydrogenase
11Sox-2SRY (sex determining region Y)-box 2
12NRSF/RESTNeural restrictive silencing factor
13NaChIISodium channel, voltage-gated, type II
14βIII-TubulinNeuro-specific tubulin, microtubules component
15CalbidinVitamin D-dependent calcium-binding protein
16BDNFBrain-derived neurotrophic factor
17GFAPGlial fibrillary acidic protein, protein specific for astrocytes in CNS
18APPAmyloid Precursor Protein
19Neurogenin 1–3Neurogenins, family of the transcription factors involved in the neuronal differentiation