Gender-Specific Neuroimmunoendocrine Response to Treadmill Exercise in 3xTg-AD Mice
Brain pathology in male and female 3xTg-AD sedentary mice (TgEXE) is partially protected against by treadmill running exercise (TgSED). (a) Representative microphotographs of hippocampus CA1 and basolateral amygdala showing amyloid-β and PHF-tau immunoreactivity using antibodies 4G8 and AT180, respectively. 4G8 reacts against amino acids 1–17 of amyloid β. AT180 detects phosphorylated tau at the threonine 321 residue. No changes in intraneuronal amyloid β or PHF-tau were induced by exercise. Scale bar = 20 m. (b) Levels of soluble amyloid β 40 (Aβ40) and amyloid-β 42 (Aβ42) in cerebral cortex as determined by sandwich ELISA. Treadmill exercise induced a protective change by decreasing the Aβ42/Aβ40 ratio (Aβ42/40) (ANOVA, ). Values are the mean ± SEM, . Statistics: .
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