cNEUPRO: Novel Biomarkers for Neurodegenerative Diseases

Box 3

Preanalytic standard operating procedures for CSF and blood.

Preanalytical standard operating procedure for CSF:

(i) Cerebrospinal fluid (CSF) is collected by lumbar puncture (LP). Ventricular CSF can also be included

   but should be clearly labelled as such.

(ii) CSF is collected in polypropylene tubes.

(iii) A standardised volume (10–12 mL) is collected.

(iv) Samples contaminated with more than 500 red blood cells/$\mu$L should not be included.

(v) Collected CSF is centrifuged at approximately 1,000–2,500 x g at +$4^{°}$C or room temperature for 10

   minutes within 1 hour after the sampling.

(vi) The supernatant is pipetted off, gently mixed to avoid possible gradient effects and aliquoted in

      portions in polypropylene tubes.

(vii) The samples are stored at $-$8$0^{°}$C without having been thawed and re-frozen.

Preanalytical standard operating procedure for serum and plasma:

(i) Serum and plasma are collected by vein puncture.

(ii) Plasma is collected into polypropylene tubes containing EDTA.

(iii) Serum is collected into polypropylene tubes without additives.

(iv) Collected blood samples are centrifuged at approximately 1,000–2,500 x g at +$4^{°}$C or room

      temperature for 15 minutes within 2 hour after the sampling.

(v) The supernatant is pipetted off, gently mixed to avoid possible gradient effects and aliquoted in

    portions in polypropylene tubes.

(vi) The samples are stored at $-$8$0^{°}$C without having been thawed and re-frozen.