Grape-Seed Polyphenolic Extract Improves the Eye Phenotype in a Drosophila Model of Tauopathy
Figure 2
GSPE does not inhibit Gal4-mediated expression of GFP. (a) Western-blot analysis of lysates from TubGal4 GFP flies (created by crossing TubGal4 flies to UAS-GFP flies) reared from egg deposition either on food supplemented with water (vehicle control) (Lanes 1–3) or reared on GSPE food (lanes 4–6). Each lysate was prepared from 6 fly heads, and three independent groups of 6 flies per group (lane) are shown. The same immunoblot was probed first with antibodies raised against GFP (upper panel) and then immunoblots were stripped and reprobed with antiactin antibodies (lower panel). (b) GMR-grim flies have very small, almost absent eyes when reared on control food. (c) Eye size is not affected when GMR-grim flies are reared on GSPE food.
