Choline Modulation of the Aβ P1-40 Channel Reconstituted into a Model Lipid Membrane
Figure 4
AβP1-40 channel activity in OxCh PLM in the absence and in the presence of different choline concentrations. Experiments were performed on an OxCh PLM in the presence of different concentrations of choline added or in the presence of high choline concentrations on the cis-side of the medium; the applied voltage was set at 60 mV; the arrows indicate the choline additions when the AβP1-40 channel fluctuates in the open state. The channel central conductance (nS ± SE)/frequency (events/min ± SD) without and with choline added to the medium was: AβP1-40 = 0.161 ± 0.008/7.73 ± 0.34; AβP1-40 + choline (5×10−11 M) = 0.215 ± 0.007/15.97 ± 0.53; AβP1-40 + choline (1×10−10 M) = 0.179 ± 0.004/10.56 ± 0.40; AβP1-40 + choline (1.5×10−8 M) = 0.168 ± 0.001/11.85 ± 0.60. The number of channel considered was: AβP1-40 = 505; AβP1-40 + choline (5×10−11 M) = 898; AβP1-40 + choline (1×10−10 M) = 652; AβP1-40 + choline (1.5 ×10−8 M) = 358. Amplitude histograms of channel conductance for each trace are shown below traces.
