Effects of Immunomodulatory Substances on Phagocytosis of A by Human Microglia
Figure 4
Effects of Protollin (white boxes) and interleukin-1β (IL-1β) and interferon-γ (IFNγ) (grey boxes) on the uptake of Aβ 1–42 in human CHME3 microglial cells. The cells were incubated with 1 g/mL Aβ 1–42 following prestimulation for 24 hours with Protollin at 0.001–1 g/mL, or 50 ng/mL IL-1β and 50 ng/mL IFNγ. The cells were harvested at 24, 48, 72, and 96 hours after addition of Aβ 1–42. The data are expressed as % uptake of control Aβ 1–42 set at 100%, and shown as median ± percentiles (25%–75% and 10%–90%), (Protollin) or (cytokines) for 24 and 48 hours, and (Protollin) of (cytokines) for 72 and 96 hours. A statistically significant effect of treatment was found by Kruskal-Wallis ANOVA at 96 hours when incubating microglia with Protollin and at 72 hours when incubating with IL-1β and/or IFNγ . Statistical difference from control is indicated by , , and indicates statistical difference between IL-1β and IL-1β IFNγ.
