Centella asiatica Attenuates D-Galactose-Induced Cognitive Impairment, Oxidative and Mitochondrial Dysfunction in Mice

Table 3

Effect of Centella asiatica (CA; 150 and 300 mg/kg, p.o.) on D-gal-induced oxidative stress parameters in rat brain.

Treatment (mg/kg)	MDA levels nmol MDA/mg of protein (% of control)	Nitrite levels μmol/mg of protein (% of control)	Non protein Thiol nmol/mg of protein (% of control)	Catalase μmol of hydrogen peroxide decomposed/min/mg of protein (% of control)	Superoxide dismutase Units/mg of protein (% of control)	Glutathione-S-transferase nmol of CDNB conjugated/min/mg of protein (% of control)
Naïve	0.1768 ± 0.04 (100)	216.8 ± 30.06 (100)	0.0653 ± 0.005 (100)	0.709 ± 0.04 (100)	48.252 ± 3.19 (100)	97.9 ± 5 (100)
D-gal (100)	0.5965 ± 0.05^a (338.63)	605.83 ± 28.32^a (279.44)	0.0256 ± 0.003^a (39.203)	0.147 ± 0.018^a (20.73)	13.873 ± 2.11^a (28.75)	37.8 ± 5.3^a (38.61)
CA (300)	0.172 ± 0.03 (97.82)	218 ± 26.7 (100.55)	0.0648 ± 0.004 (99.23)	0.703 ± 0.03 (99.15)	47.22 ± 3.111 (97.86)	99.9 ± 5.2 (102.04)
CA (150) + D-gal (100)	0.486 ± 0.03^b (247.88)	456 ± 16.2^b (210.33)	0.041 ± 0.001^b (62.78)	0.309 ± 0.023^b (43.58)	21.93 ± 2.049^b (45.44)	47.67 ± 2.3^b (48.69)
CA (300) + D-gal (100)	0.279 ± 0.04^b,c (157.8)	353.83 ± 18.6^b,c (163.2)	0.051 ± 0.001^b,c (78.1)	0.427 ± 0.018^b,c (60.22)	35.872 ± 2.05^b,c (74.34)	64.6 ± 1.8^b,c (65.98)

Values are mean ± S.E.M. ^a𝑃<.05 as compared to naive group; ^b𝑃<.05 as compared to D-gal-treated group; ^c𝑃<.05 as compared to CA (150) group+ D-gal group (repeated measures one-way ANOVA followed by Tukey’s test for multiple comparisons).