Determination of soluble (SolT) and sarkosyl-insoluble (SinT) fractions from wild-type tau or the synthetic mutants tau-S409A and tau-S409E, as obtained in WT cells (a) or pho85Δ cells. (b) Western blot with tau5 of a representative experiment is shown on the left, and quantifications of SinT levels for each experiment are shown on the right. Adapted from [86] with permission from the publisher and the authors.
