Hypothesis: Soluble Aβ Oligomers in Association with Redox-Active Metal Ions Are the Optimal Generators of Reactive Oxygen Species in Alzheimer's Disease
Figure 1
ESR spectra of the •OH adduct of 5,5-dimethyl-1-pyrroline -oxide (DMPO) recorded after 48 h incubation of (a) Aβ(1–40), (b) Aβ(1–42), (c) Aβ(25–35) (intensity ) and (d) Aβ(40–1) at 37°C and all at 100 μM. The intensity of the spectra observed in (a), (b), and (c) varied with incubation time as described in the text whereas in (d) no DMPO adduct spectrum was observed after any period of incubation. Control samples gave straight line spectra, such as that illustrated in (d), at every time point examined. The experimental methodology, reaction conditions, and ESR spectrometer settings are fully detailed elsewhere [9, 16].
