Table 2: Differences between the free Gibbs energy of 5′-end and 3′-end of the duplex (resp. to the polarity of the antisense strand), calculated independently for three and for four consecutive base pairs shown for each duplex . The values of were calculated according to [60].

siRNASequence (kcal/mol) (kcal/mol)
(for 3 bp)(for 4 bp)

P15′-GUGG—UCAGTT-3′11.6
3′-TTCACC—AGUC-5′
P25′-UGUG—CUCATT-3′−0.2−0.1
3′-TTACAC—GAGU-5′
P3  5′-UUGU—CCUCTT-3′−1.9−2.7
3′-TTAACA—GGAG-5′
P4  5′-GUUG—GCCUTT-3′−0.5−1.8
3′-TTCAAC—CGGA-5′
P5   5′-UGUU—AGCCTT-3′−3−3.8
3′-TTACAA—UCGG-5′
P65′-GUGU—AAGCTT-3′−1.20
3′-TTCACA—UUCG-5′
P75′-AGUG—AAAGTT-3′0.71.6
3′-TTUCAC—UUUC-5′
P8  5′-CAGU—UAAATT-3′1.72.7
3′-TTGUCA—AUUU-5′
P95′-ACAG—GUAATT-3′1.41
3′-TTUGUC—CAUU-5′
P10  5′-UACA—GGUATT-3′0−1.1
3′-TTAUGU—CCAU-5′
P11  5′-CUAC—UGGUTT-3′−1.7−1.4
3′-TTGAUG—ACCA-5′
P125′-UCUA—UUGGTT-3′−0.7−0.5
3′-TTAGAU—AACC-5′
P135′-UUCU—GUUGTT-3′0.50.1
3′-TTAAGA—CAAC-5′
P14    5′-UUUC—GGUUTT-3′−0.7−1.3
3′-TTAAAG—CCAA-5′
P15   5′-UUUU—UGGUTT-3′−2.7−3.6
3′-TTAAAA—ACCA-5′
P165′-AUUU—GUGGTT-3′−3.4−4.6
3′-TTUAAA—CACC-5′
P17  5′-CAUU—UGUGTT-3′−1.2−2.1
3′-TTGUAA—ACAC-5′
P18  5′-UCAU—UUGUTT-3′0.70.7
3′-TTAGUA—AACA-5′
P19  5′-UUCA—GUUGTT-3′0.50.2
3′-TTAAGU—CAAC-5′