Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 3
Correlation between Aβ-FAM binding and surface PtdSer observed on cells sorted on the basis of annexin V-PE binding. PC12 cells were exposed to annexin V-PE for 20 min, and sorted based on the levels of annexin V-PE binding. The histograms in (a) show the results of the distribution of the cells. The numbers in the figures are the averaged percentage of PtdSer− and PtdSer+ cells from four sorting experiments. The resulting groups of cells, untreated control, PtdSer+, and PtdSer- were then treated with Aβ-FAM. (b) shows the fluorescence histograms on the basis of Aβ-FAM binding. The results of cell count in the 1 gate, Aβ-FAM positive cells, show that the PtdSer+ group of cells has the highest percentage (88%) of cells with bound Aβ.
