Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 4
Specific antibody to PtdSer reduces the number of surface membrane binding sites for Aβ-FAM. PC12 cells were exposed for 20 min to annexin V-PE, for PtdSer identification, and for 6 hrs to Aβ-FAM to study Aβ-FAM binding before and after treatment with anti-PtdSer monoclonal antibody (Anti PtdSer mAb). The fluorescence histograms in (a) show that cells treated with anti-PtdSer mAb reduced the percentage of PtdSer+ cells in gate 2 by 42% of the untreated value. The fluorescence histograms in (b) show that the combined treatment of anti-PtdSer mAb and Aβ-FAM reduced the percentage of PtdSer+ cells in gate 2 by 26% of the value observed after treatment with Aβ-FAM alone. The fluorescence histograms in (c) show that Aβ-FAM binding, measured as Aβ + cells in 1, is also reduced, by 36%, when cells are pretreated with anti-PtdSer mAb. The tables in each panel show the percentage of PtdSer+ and Aβ + cells in gates 2 and 1, respectively and Δ% represents the percentage of the change.
