Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 5
Correlation between the levels of cholesterol in the membrane and the binding of Aβ-FAM to the membrane surface. PC12 cells were exposed for 80 minutes to Aβ42-FAM, washed to remove unbound Aβ-FAM, and then incubated for 40 minutes in PBS containing Filipin III. (a) shows the flow cytometric analysis of the cholesterol content of the three subpopulations of cells distinguished on the bases of the level of Aβ-FAM binding. The percentage of cells and the Geo mean of fluorescence (GeoMF) values measured within the gate are displayed in the bottom table. The highest level of filipin III fluorescence is observed in the cells from the subpopulation C, defined as extra high Aβ-FAM affinity. The bars plot in (b) summarizes the percentage of cells and the GMF values within gate M corresponding to each subpopulation of data pooled from four experiments.
