Fluorescent Analysis of the Cell-Selective Alzheimer's Disease Aβ Peptide Surface Membrane Binding: Influence of Membrane Components
Figure 6
Correlation between Aβ-FAM binding and the levels of membrane cholesterol observed in cells sorted on the basis of the Aβ-FAM fluorescence. PC12 cell and hippocampal neuronal cells were exposed for 80 minutes to Aβ-FAM for binding and then sorted on the basis of the FAM fluorescence. After sorting Aβ-FAM, treated cells were pooled into two populations: no Aβ-FAM affinity and Aβ-FAM affinity. Cells were cultured and allowed to grow and membrane cholesterol levels were measured at 1 and at 8 days after sorting. (a) and (b) show the results for the PC12 cell and hippocampal neuronal cells, respectively. The correlation between the levels of Aβ-FAM binding and the levels of membrane cholesterol is observed even after 8 days of cell sorting.
