Effect of Metal Chelators on <i>γ</i>-Secretase Indicates That Calcium and Magnesium Ions Facilitate Cleavage of Alzheimer Amyloid Precursor Substrate

<table>Effect of Ca<sup>2+</sup> and Mg<sup>2+</sup> on <i>γ</i>-secretase activity. (a) The effects of EDTA (2 mM) and EGTA (2 mM) were compared in assays carried out with <i>γ</i>-secretase preparations from guinea pig or mouse brains. (b) Quantitative analysis of the blot shown in (a). (c) <i>γ</i>-secretase assay with guinea pig brain activity was carried out in HEPES buffer plus 150 mM KCl, in the presence of increasing CaCl<sub>2</sub> or MgCl<sub>2</sub> concentrations as indicated.</table>

International Journal of Alzheimer’s Disease

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Figure 4

Figure 4: Effect of Metal Chelators on <i>γ</i>-Secretase Indicates That Calcium and Magnesium Ions Facilitate Cleavage of Alzheimer Amyloid Precursor Substrate 

Figure 4 | Effect of Metal Chelators on γ-Secretase Indicates That Calcium and Magnesium Ions Facilitate Cleavage of Alzheimer Amyloid Precursor Substrate 