Cell Surface Binding and Internalization of Aβ Modulated by Degree of Aggregation

Figure 4

Confocal Microscopy images showing Aβ time course of cell entry. Confocal microscopy images of differentiated PC12 cells treated with various Aβ samples exposed to endosomal conditions after 1, 6, and 24 hours. All scale bars are 20 μm in length. Aβ40²⁴ associates rapidly to differentiated PC12 compared to Aβ40⁰, whereas Aβ40⁹⁰ displays weak cell association over its treatment course. Aβ42²⁴ displays rapid internalization compared to Aβ42⁰. Aβ42⁹⁰ displays reduced cell interaction compared to Aβ42⁰ and Aβ42²⁴.