A Changing Perspective on the Role of Neuroinflammation in Alzheimer's Disease
Figure 2
Stimulation of BV2 cultured microglial cells to polarize the response to M1, M2a, M2b, or M2c. BV2 microglial cells were cultured in normal DMEM media. When confluent, cell media was changed to serum-free DMEM media for 24 hours. Then media was changed to either DMEM plus IFNγ (2.5 ng/mL) to stimulate an M1 response (a), DMEM plus IL-4 (20 ng/mL) and IL-13 (20 ng/mL) to stimulate an M2a response (b), DMEM plus immune complexes prepared as described in [32] (5 μg/mL Aβ coated with IgG) to stimulate an M2b response (c), DMEM plus IL-10 (10 ng/mL) to stimulate an M2c response (d), or DMEM alone to act as an untreated control. Cells were then harvested 12 hours after the start of treatment. We repeated the experiments 3 times, each on separate cultures of different passage numbers. Data are shown as fold change compared to untreated control BV2 cells. and .
