Microglial Amyloid-1-40 Phagocytosis Dysfunction Is Caused by High-Mobility Group Box Protein-1: Implications for the Pathological Progression of Alzheimer’s Disease
Figure 4
A degradation with the microglial cytosolic fraction. (a) A40 and A42 were incubated with the rat microglial cytosolic fraction for 0, 6, and 24 h. After incubation, samples were subjected to Western blot analysis using the anti-A antibody, and the amount of A was semiquantitatively measured. **, *** versus time point 0 h. †††versus A40 at time point 24 h. (b) A40 and A40 with HMGB1 were incubated with rat microglial cytosolic fractions for 0, 6, and 24 h. After incubation, the samples were subjected to Western blot analysis using the anti-A antibody, and the amount of A was semiquantitatively measured. **, *** versus A40 alone at each time point.