Expression of Phenotypic Astrocyte Marker Is Increased in a Transgenic Mouse Model of Alzheimer’s Disease versus Age-Matched Controls: A Presymptomatic Stage Study

Figure 1

APP and TNFα expression in the hippocampus of 3-month-old APPswe/PS1dE9 mice. (a) APP (112 kDa) and (b) TNFα (51 kDa) expressions were determined by Western blotting. Equal protein loading was ascertained by the parallel assessment of β-actin (42 kDa) and results were expressed as the relative optical density (ROD). (c) In APPswe/PS1dE9 mouse group, the increase in APP correlates positively with TNFα (; ). Correlation analysis between respective ROD values as determined in Western blot studies (a and b) was performed by linear regression. (d) The level of TNFα was also assayed by ELISA and expressed in pg/mL protein. Each depicted point corresponds to the individual quantitative measures obtained for each WT () and APPswe/PS1dE9 () per genotype group; indicates . NTg: wild type; Tg: APPswe/PS1dE9.

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