Multiple Patterns of FHIT Gene Homozygous Deletion in Egyptian Breast Cancer Patients
Figure 1
FHIT gene exons are subjected to homozygous deletions in Egyptian neoplastic and preneoplastic breast tissues. DNA was extracted from breast cancer and matched normal tissue samples then subjected to PCR analysis of FHIT exons. (a) Amplification of exons 3, 4, 5, 6, 7, 8, and 9. β-actin was used as a control for equal amount of DNA used in the amplification. Intensity of amplified band in tumour (T) samples was compared with its relative one in normal sample (N). Samples that showed significant reduction or loss of the amplified exons were considered positive for exon homozygous deletion (HD). (b) Cumulative data of HD in individual patients. (C) Incidence of HD of FHIT exons in breast cancer patients. (c) Incidence of HD of FHIT exons in breast cancer patients. (d) Association between HD of FHIT exons and breast cancer patients’ clinicopathological parameters.
