Multiple Patterns of FHIT Gene Homozygous Deletion in Egyptian Breast Cancer Patients

Table 2

Sequence of primers used in this study.

Exon	Sequence	Expected size (BP)
Exon 3	3F: 5′ AGGGTGATACTAGCTGCTTT 3′	284
	3R: 5′ TGACTTTAGCCAGTGGCA 3′
Exon 4	4F: 5′ TTGTACCTAGAGCCATCTGG 3	224
	4R: 5′ GGATACTCACAGCAGGTCAA 3′
Exon 5	5F: 5′ TGAGGACATGTCGTTCAGATTT 3′	255
	5R: 5′ CTGGTGTCTCCGAAGTGGGAGGG 3′
Exon 6	6F: 5′ ATGTTCTTGTGTGCCCGCTGCGGCCAGT 3′	147
	6R: 5′ CTGCATGGAAAAGGTGAGAGAGGTCCCATG 3′
Exon 7	7F: 5′ TGGTCCCCATGAGAATACTATAAATTAACA 3′	305
	7R: 5′ TTACGGCTCTAACACTGAGGGTCTCTCTGA 3′
Exon 8	8F: 5′ GAGTAATTGGGCTTCATGAGAGCATCACT 3′	225
	8R: 5′ AGGTTGATGTCATCCCACCGACAGT 3′
Exon 9	9F: 5′ TTCTCCAAAGCTCCAGAAACATGACAAGGA 3′	119
	9R: 5′ GTCTTTACCTGTGTCACTGAAAGTAGACCC 3′
β-actin	F: 5′ TCATCACCAATTGGCAATGAG 3′	147
	R: 5′ CACTGTGTTGGCGTACAGGT 3′