Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer

Table 1

Requirement for preparation of mammary gland.

MEC ^1	SC^2	ECM	𝑁^3	𝑥/𝑇^4	Outcome
Humanized mammary fat pads
MCF7 (2 × 10^5)	hTert MSC (2 × 10^5)	Col I plus matrigel (3 : 1)	8	0/16	Solid tumor
MCF7/CEACAM1-4S (2 × 10^5)	hTert MSC (2 × 10^5)	Col I plus matrigel (3 : 1)	8	14/16*	Differentiation into striated epithelium
Normal mouse mammary fat pads
MCF7 (2 × 10^5)	hTert MSC (2X10^5)	Col I plus matrigel (3 : 1)	8	0/16	Solid tumor
MCF7/CEACAM1-4S (2 × 10^5)	hTert MSC (2X10^5)	Col I plus matrigel (3 : 1)	8	14/15*	Differentiation into striated epithelium

¹Mammary epithelial cell line (number of cells injected), ²Stromal cell line (number of cells injected), ³Number of animals, ⁴Number of xenografts exhibiting striated epithelial structure (𝑥)/Total number of xenografts (𝑇), *indicates statistical significance (𝑃<.01) for differentiation of xenografts, when compared with control group (MCF7).