The Role of Aryl Hydrocarbon Receptor and Crosstalk with Estrogen Receptor in Response of Breast Cancer Cells to the Novel Antitumor Agents Benzothiazoles and Aminoflavone

Figure 10

Cytotoxicity of aminoflavone in vitro and in vivo. (a) Selective cytotoxicity of aminoflavone to MCF-7 breast tumor cells. Cell lines were seeded into 24-well plates and allowed to grow for 48 hours. Cells were treated with 10⁻¹⁰ to 10⁻⁵ mol/L aminoflavone for an additional 72 hours. Cell monolayers were stained with sulforhodamine (b) and protein was determined spectrophotometrically. Points, mean ± SD (𝑛=10). SD was <5% for all drug concentrations and was omitted for purpose of graphical clarity. (b) Antitumor activity of aminoflavone against MCF-7 breast tumor xenografts. Treatments were given on a QD × 5 schedule beginning on day 13. There were 20 mice in the vehicle control group and 6 mice per dose of aminoflavone in the treated groups [19].

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