Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines
Figure 4
Effect of berry extracts and polyphenols on the expression of nuclear receptors and cell-death markers in ICI-sensitive (LCC1) Cell Lines. Biomarker analysis results from 3 d treatment of berry extracts and polyphenols. Data represented as fold change over the vehicle. Extract only treatment was normalized to nontreated cells (first lane). Extract +ICI was normalized to the appropriate vehicle treatment (first lane). Vehicle treatment contained 0.01% EtOH and 0.1% DMSO. Concentrations of treatments were as follows: ICI = 1 nM; EJAE = 0.1 μg/mL; RRE = 1 μg/mL; EA = 1 μM; DEL = 1 μM. Concentration of extract used showed the most significant reduction in cell proliferation of LCC1 cells after 6 d treatment. Each extract or polyphenol used for treatment was diluted 1000-fold from stock concentration. *designates a significance of compared to vehicle control.
