International Journal of Breast Cancer / 2012 / Article / Fig 4

Research Article

A Comparison of Cholesterol Uptake and Storage in Inflammatory and Noninflammatory Breast Cancer Cells

Figure 4

Breast cancer in vitro invasion assays. (a) ORO staining of treatments prior to invasion assay for SUM149, MDA-MB-231, and MCF10a cells, respectively. (b) Representative images of invasion assays. Cells were grown in medium containing FBS (untreated control), LPDS, LPDS with addition of Atorvastatin (A + LPDS) and LPDS with the addition of LDL (LDL + LPDS), resuspended in serum-free medium, placed on a Matrigel coated filter with 8 μ pores and allowed to invade towards a chemoattractant for 22 h. Invaded cells were stained with crystal violet and images taken using a Nikon Eclipes TE-200U. Scale bars represent 50 μm. (c) Quantitation of invaded cells relative to untreated controls for SUM149, MDA-MB-231, and MCF10a cells, respectively (* ). All experiments were performed at least three separate times.

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