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International Journal of Breast Cancer
Volume 2012 (2012), Article ID 412581, 10 pages
http://dx.doi.org/10.1155/2012/412581
Research Article

A Comparison of Cholesterol Uptake and Storage in Inflammatory and Noninflammatory Breast Cancer Cells

Department of Biological Sciences and The Center for Translational Cancer Research, The University of Delaware, 320 Wolf Hall, Newark, DE 19716, USA

Received 7 August 2012; Revised 7 December 2012; Accepted 7 December 2012

Academic Editor: Claudio Luparello

Copyright © 2012 Breonna J. Martin and Kenneth L. van Golen. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1: is a representative phase contrast image showing the presence of MCF10a cells in samples that were Oil Red O stained. Lack of staining makes them barely visible in the bright field image (Figure 1A). The scale bar represents 10 μm.

Supplementary Figure 2: is Filipin staining of breast cancer cell lines. The stain intensity of the MCF10A, SUM149 and MDA-MB-231 cells was determined using LSM Image Examine. SUM149 and MDA-MB-231 cells display a significantly lower staining intensity than MCF10a cells (p<0.01, error bars are S.E.M), using One-way ANOVA followed by Tukey's HSD test (B). All experiments were performed at least three separate times.

  1. Supplementary Figure 1
  2. Supplementary Figure 2