Research Article
Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
Table 1
Different characteristics between the five HER2 genetic assays.
| ā | Dako HER2 FISH | Dako HER2 IQ-FISH | Dako HER2 CISH | ZytoVision HER2 FISH | ZytoVision HER2 CISH |
| Gene | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 | HER2 | CEN17 | Probe | DNA | PNA | DNA | PNA | DNA | PNA | DNA | DNA | DNA | DNA | Label color | TxRed | FITC | TxRed | FITC | Red | Blue | FITC | Rhodamine | Green | Red | Blocking reagent | alu-PNA | alu-PNA | alu-PNA | Repeat free | Repeat free | Visualization | Fluorescence | Fluorescence | Chromogenic | Fluorescence | Chromogenic | Hybridization reagent | Formamide | Ethylene carbonate | Formamide | Formamide | Formamide |
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CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization; PNA: peptide nucleic acids.
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