Research Article
Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
Table 3
Concordance between one analysis against the mean result of the other four assays used.
| (a) |
| | Consensus (mean of the other four assays) | CISH ZytoVision
| | Amplified (≥2.0) | Nonamplified | Total |
| | Amplified (≥2.0) | 18 | 1 | 19 | | Nonamplified | 1 | 75 | 76 | | Total | 19 | 76 | 95 |
|
|
Concordance, 97,9%; Cohen coefficient, 0,9342.
|
| (b) |
| | Consensus (mean of the other four assays) | CISH Dako | | Amplified (≥2.0) | Nonamplified | Total |
| | Amplified (≥2.0) | 18 | 1 | 19 | | Nonamplified | 0 | 76 | 76 | | Total | 18 | 77 | 95 |
|
|
Concordance, 99,0%; Cohen coefficient, 0,9664.
|
| (c) |
| | Consensus (mean of the other four assays) | FISH ZytoVision
| | Amplified (≥2.0) | Nonamplified | Total |
| | Amplified (≥2.0) | 18 | 1 | 18 | | Nonamplified | 1 | 76 | 77 | | Total | 19 | 76 | 95 |
|
|
Concordance, 97,8%; Cohen coefficient, 0,9664.
|
| (d) |
| | Consensus (mean of the other four assays) | FISH Dako
| | Amplified (≥2.0) | Nonamplified | Total |
| | Amplified (≥2.0) | 18 | 0 | 18 | | Nonamplified | 2 | 75 | 77 | | Total | 20 | 75 | 95 |
|
|
Concordance, 97,9%; Cohen coefficient, 0,9343.
|
| (e) |
| | Consensus (mean of the other four assays) | IQ-FISH Dako
| | Amplified (≥2.0) | Nonamplified | Total |
| | Amplified (≥2.0) | 17 | 1 | 18 | | Nonamplified | 0 | 77 | 77 | | Total | 17 | 78 | 95 |
|
|
Concordance, 99,0%; Cohen coefficient 0,9650.
CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization.
|
