Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines

Table 1

Nucleotide sequences of the primer pairs and probes used to amplify and detect TPM2 RNAs.

TPM1.Exon 1a (+): 5′-GCTCCTGCTGCAGCCCCAGG-3′
TPM1.Exon 9b (−): 5′-CTTCTGTACAATAGAAAGCA-3′
TPM1.Exon 9d (−): 5′-TTCACATGTTGTTTAACTCCAGT-3′
TPM1.Exon 2a (probe): 5′-GAA GTT GCT GCG GGT GTC GG-3′
TPM1.Exon2b (probe): 5′-AAG ATG AAC TGG ACA AAT AC-3′
TPM1.Exon 3 (probe): 5′-ATCGTGCCCAGGAGCGTCTGGCAA-3′
TPM1.Exon 6a (probe): 5′-GAAAGCATTAATGGCTGCAGAGG-3′
TPM1.Exon 6b (probe): 5′-TGAAGTCACTGGAGGCTCAGG-3′
TPM1.Exon 9a (−) (probe/prim) 5′-ATGTCGACCTTATATGGAAGTCATATCG-3′
GAPDH. P1(+): 5′-GTTTACATGTTCCAATATGATTCCAC-3′
GAPDH.P2(−): 5′-TCATATTTGGCAGGTTTTCTAGA-3′
GAPDH.Probe: 5′-GTGGAGTCCACTGGCGTCTT-3′
TPM2.Exon 1a(+): 5′-ATGGACGCCATCAAGAAGAA-3′
TPM2.Exon 9d(−): 5′-TGGGGCTGGCCCTCACAGGTT-3′
TPM2.Probe: 5′-AGAGGGCTGAGGTGGCCGAGAGCCG-3′