International Journal of Breast Cancer

Research Article

Expression of Tropomyosin 1 Gene Isoforms in Human Breast Cancer Cell Lines

Table 2

Tropomyosin 1 gene RNA isoform expression in breast cancer cell lines.
Cell lineRNA isoform expression▲
TMP1TPM1TPM1TPM1TMP1TPM1TPM1TPM1TPM1TPM1TPM1TPM1TPM1νTPM1ξGAPDH
Breast cancer               
 HCC 1143−++++++−−−−−−−++−++++
 HCC 1187−++++++−−−−−−+++−−++++
 BT 474−++++++−−−−−−+−−−++++
 mDAMB 157−++++++−−−−−−++++−++++
 HCC 1806−−+++−−−−−−−++−++++
 HCC 1419−+++++−−−−−−+−+−++++
 mDAMB 453−−+++−−−−−−++−+−++++
 mDAMB 468−++++++−−−−−−+++−++++
 MCF7−−++++−−−−−−++−−−++++
MCF10A (normal breast)−++++++−−−−−−−+++++++
HCC 1143 (BL)+−−−−−−−−−−−−−−++++
HCC 1143 (BL)+−−−−−−−−−−−−−−++++
Adult heart++++−−−−−−−−+−−−−++++
Fetal heart+++−−−−−−−−++−−−−++++
▲ = cell lines were analyzed by RNA-PCR for the indicated TPM1 isoforms. GAPDH RNA was analyzed as a control for baseline RNA expression. Expression was qualitatively recorded as either negative (−) or plus (+) to four pluses (++++).
= primary tumor; the rest were metastatic tumors.
+BL = B-lymphocyte cell lines.