Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations

Figure 4

Sema3A alters cell morphology on collagen and fibronectin but has minimal effects on laminin. (a) Example images of cells stained with TRITC-phalloidin. Rounded cells display circularity close to 1 and a low aspect ratio (close to 1), while more elongated cells have circularity close to 0 and a high aspect ratio (greater than 1). Scale bar = 20 μm. Cell circularity and aspect ratio were measured from cells exposed to substrata coated with increasing concentrations of collagen ((b) and (c)), fibronectin ((d) and (e)), and laminin ((f) and (g)). Sema3A consistently reduced circularity and increased aspect ratio on substrata coated with increasing concentrations of collagen, while the effects of Sema3A on fibronectin and laminin were less consistent. Graphed data represent the mean ± SEM from at least 80 cells for each condition. , , and indicate statistical difference relative to IgG1 Fc control; two-sample -test.

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